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Beer Fermentation and CO2

How Does It Work?
The next time you see a beer commercial or drink a cold draft, take the time to appreciate the importance of carbon dioxide in the brewing process.
In The Beginning
Beer starts out as wort, a mix of water and natural grains. When Brewer’s yeast (a fungi) is added to the mix, it "eats" the starches and sugars in the wort while giving off alcohol and carbon dioxide (CO2) gas.
It is the infinitely variable combinations of water, grains, yeast, alcohol levels and the amount of carbonation which give each beer its unique color and taste.
Fermentation
During fermentation, the CO2 given off by the yeast was typically vented into the air. However, modern breweries now use CO2 capture systems. Once fermentation is complete, the brewing vats are sealed to build pressure and give the beer natural carbonation.
To measure the CO2 levels before and during production, brewers use our Multi Gas Sampling Data Logger. These meters may also be used to spot check CO2 levels inside vats before workers go inside to clean them.
Bottling
During bottling, CO2 gas is used to pre-fill each bottle before the beer is added. This process minimizes exposure to oxygen, reduces foam, and maintains the CO2 in the beer before it is capped. In commercial breweries, even the head-space (air below the cap in the bottle) is replaced with CO2 to keep out oxygen so that the bottled beer can last longer in non-refrigerated stores. In order to test CO2 during bottling, a high-speed sensor like our SprintIR Fast CO2 sensor is used, which can measure CO2 levels 20 times per second.
One of the questions we get asked is "Do you have a sensor you can put INTO the beer to measure the CO2 concentration?
The short answer is that it does not exist. The longer answer is that several companies make devices or systems that measure the pressure differential in carbonated beer to calculate CO2. But they don't measure CO2 directly.
Our ambient air CO2 sensors use Henry’s Law, which states:
“The principle that at a constant temperature the concentration of a gas dissolved in a fluid with which it does not combine chemically is almost directly proportional to the partial pressure of the gas at the surface of the fluid.”
Using Henry’s Law, you can measure the CO2 level in a liquid by measuring the CO2 in the head space above the liquid, then compute the actual CO2 level. Since the bottles are filled with CO2 before they are filled with liquid - and because CO2 is heavier than air - a high-speed sensor testing each empty bottle is full of CO2 before filling is a perfect solution.

For most beer sold in kegs, CO2 from pressurized storage tanks are used to force the beer out of the tap. To protect customers and employees that work around pressurized CO2, breweries use our Carbon Dioxide (CO2) Fixed Gas Detector w/ Remote Sensor (0-5%). Because of the increasing use of high-pressure CO2 systems, it has become one of the most popular products we sell.
More Than CO2
Interestingly, not every beer relies on CO2 for bubbles. The secret of Guinness Stout's creamy taste is its mix of nitrogen and carbon dioxide rather than pure CO2. The smaller nitrogen bubbles produce a smoother head. At taverns that serve Guinness on tap, they use a special nozzle that aerates the stout with nitrogen as it's poured.
In cans of Guinness this is duplicated by inserting a capsule that releases pure nitrogen when the can is opened.
CO2Meter Supports CO2 Safety In Breweries
CO2Meter has many different options available to brewers in regards to CO2 safety, whether it be a personal safety device, or a wall mounted/fixed devices, we have many products available to brewers in order to keep themselves, employees and patrons safe, while also staying compliant to the local fire codes, standards and regulations. Contact Us Online today or call us at 877.678.4259 to make your establishment CO2 safe.
Why Control CO2 Levels at Night in Plant Grow Rooms?
In this article, we outline 5 reasons why you may consider turning off the CO2 supply at night via your CO2 monitor and controller.
How to Fix GasLab® Software Not Detecting Your CO2 Sensor
Having trouble connecting your CO2 sensor to GasLab® Software? Learn how to fix driver issues, install the correct FTDI USB-to-UART drivers, and restore communication.
Temperature Change Impacts Oxygen Sensors

If you work with gases like we do at CO2Meter, you already know that the ideal gas law PV=nRT shows that absolute gas concentration decreases as temperature increases. This relationship is important to anyone measuring oxygen levels during combustion, fermentation, distillation or algae production.
But like they say, “a picture is worth a thousand words.”
That’s what we discovered when we measured the change in oxygen gas levels in a sealed environment using our UVFlux 25% Oxygen sensor and Gaslab software. Using the built-in RS232 protocol, the UVFlux sensor samples both the oxygen level and the temperature inside the sensor once per second.
Over several minutes we raised the temperature of the sample from 24C to 34C, and were able to see in real-time the oxygen concentration in the sample drop from 20.6% to 19.8%.
click image on right for larger view.
Note the internal sensors components are corrected for operating temperature over the range. No compensation is made for the temperature of the gas being measured. Also note the change in oxygen level measured slightly lagged behind the change in temperature.
In addition to temperature, the % relative humidity will have an impact on the oxygen concentration in a sample. At the same pressure and temperature, a volume of oxygen at 100% humidity will contain less oxygen molecules than if there were no water.
Constant CO2 Level in Greenhouse
Controlled CO2 gas inflow and improved air mixture can help maintain optimum CO2 levels in small indoor greenhouses or grow rooms. These were the lessons learned after working with a client who was attempting to control the CO2 levels in a group of 3 10x20” greenhouses.
The client wanted to maintain different consistent CO2 levels in each greenhouse to test the effects on the plants inside. To control the CO2, he used our iSense 1% CO2 Level Controller, a more industrial version of our Day Night CO2 Monitor & Controller for Greenhouses.
In one test greenhouse, the CO2 controller was set to turn on a CO2 Tank Regulator with Solenoid Valve at 700ppm and turn it off at 1,400ppm. While the controller correctly turned the regulator on at 700ppm, the CO2 levels were overshooting 1,400ppm before switching off the solenoid on the regulator. As a result, the clients was seeing wild swings of CO2 levels instead of the consistent level required for his experimentation.
After looking at his CO2 levels vs. time graphs, we noticed a pattern of a quick rise in the level of CO2, followed by slow drops. This lead to the conclusion that the greenhouse was being quickly flooded with CO2 before the CO2 sensor in the controller had a chance to react to the change in CO2 levels.
In other words, the problem was being caused by inefficient air mixture in an enclosed area.
In a perfect world the CO2 flow rate could be set to exactly match the CO2 loss. However, in the real world, minimum and maximum level switches are required to turn the regulator on and off to keep the CO2 levels within a specified range.
We suggested three changes to the client’s setup to help solve his problem.
- Increase air circulation with fans, especially ones placed near the CO2 gas inlet into the greenhouse.
- Make a CO2 delivery tube like industrial greenhouses with propane CO2 systems use. Drill small holes in a long piece of flexible tubing that runs the length of the greenhouse, then hang the tubing above the plants. Since carbon dioxide is heavier than normal air, it will naturally mix as it falls.
- This decreases the amount of CO2 released between the time the room reaches the optimum CO2 level and the controller senses it. In addition, a good regulator with flow control not only limits the amount of CO2 that is released, but it maintains a consistent level of flow as the CO2 is used and the tank pressure changes.
Using our suggestions, the client will be able to maintain a more consistent CO2 level in the test greenhouses in order to complete their experiments.
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